rabbit polyclonal anti-cd69  (Millipore)

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Assessing mCD69 expression in primary mouse T cells using flow cytometry and [89Zr]-DFO-H1.2F3 uptake. A, Scheme of CD69 antibody binding: H1.2F3 monoclonal anti-CD69 binds to mCD69 on the surface of activated primary mouse T cells and other immune cells. B, Scheme of CD69 imaging: CD69 can be used as a biomarker to distinguish immunologically active tumors of mice that respond to checkpoint blockade (CBP) therapy (responders) from the tumors of mice that do not respond to therapy (nonresponders). C, Ex vivo flow cytometry analysis of CD69 expression for primary mouse T cells treated with PMA/ionomycin or untreated primary mouse T cells. D, Ex vivo [89Zr]-DFO-H1.2F3 uptake for primary mouse T cells treated with 50 ng/mL PMA and 1 μg/mL ionomycin, untreated primary mouse T cells, and CT26 cells. Uptake was measured on γ-counter and normalized to percent injected dose per million cells (% ID/106 cells). Error bars, SD.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Expressing, Flow Cytometry, Binding Assay, Imaging, Biomarker Assay, Ex Vivo, Injection

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Validating CD69 expression in a CT26 syngeneic tumor immunotherapy model using ex vivo autoradiography and IHC. A, Representative autoradiography images of whole tumor sections from responders, nonresponders, and untreated control groups. B, 20x magnified histological and IHC images of CD69 expression (NovaRED) on tumors sections from responders, nonresponders, and untreated control groups. Scale bar, 100 μmol/L.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Expressing, Ex Vivo, Autoradiography

Journal: Cancer immunology research

Article Title: Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation

doi: 10.1158/2326-6066.CIR-21-0874

Figure Lengend Snippet: Correlating CD69 IHC with immune lineage markers. A, Representative tumor sections from responder and nonresponder mice. Tumor sections were assayed for the CD69 activation marker using NovaRED, and for CD4, CD8, and CD45 lineage markers using DAB staining. Scale bar, 250 μmol/L. B to E, Quantification of the number of positively stained cells per high power field (HPF) for responder and nonresponder tumor sections, at 10x magnification. Scale bars, 250 μm. Two-tailed unpaired t test was used to compare groups., P < 0.05; **, P < 0.01; ***, P < 0.001;, P < 0.0001. n = 3 tumors per group with three measurements per HPF per tumor.

Article Snippet: To probe for CD69, a polyclonal rabbit anti-murine CD69 was used (1:50; 1 mg/mL; Bioss, catalog no. bs-2499R) followed by a goat anti-rabbit IgG amplifier Ab and an horseradish peroxidase (HRP)-conjugated horse anti-goat Ab (ImmPRESS Excel Amplified Polymer Staining Kit, Vector Laboratories, catalog no. MP-7451-15).

Techniques: Activation Assay, Marker, Staining, Two Tailed Test

Journal: Mediators of Inflammation

Article Title: Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD

doi: 10.1155/2024/6263447

Figure Lengend Snippet: SCFAs alleviate inflammatory cell activities in gut ILC2 cells. (a) t-SNE feature plots revealing profiles of eight biomarkers. (b) Arg1, ST2, Thy1, KLRG1, IL-17RB, CCR9, and CD69 protein contents, as evidenced by Western blot analysis. (c) S1P, S1P2, S1P3, S1P4, and S1P5 transcript expressions, as evidenced by qRT-PCR. (d) Lung ILC2s mRNA expression in murine COPD model treated with butyrate. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.01 (relative to control mice).

Article Snippet: The membrane was blocked with 5% nonfat milk at room temperature for 2 hr and further incubated overnight with rabbit anti-arginase1 (anti-Arg1) (Sangon Biotech, Shanghai, China), Thy.1 monoclonal antibody (Proteintech Group, Inc, Wuhan, China), ST2 monoclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit antikiller cell lectin-like receptor G1 (KLRG1) (Abcam, Cambridge, MA, USA), rabbit anti-IL17RB antibody (bioss, Bejing, China), anti-CCR9 antibody (BosterBio, USA), rabbit anti-CD69 polyclonal antibody (absin, Shanghai, China), NFIL3 polyclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit anti-GATA3 polyclonal antibody (absin, Shanghai, China), histone-H3 polyclonal antibody (Proteintech Group, Inc., Wuhan, China), rabbit anti-acetyl-histone H3 monoclonal antibody (absin, Shanghai, China), or β -actin polyclonal antibody (Proteintech Group, Inc., Wuhan, China) at 4°C.

Techniques: Western Blot, Quantitative RT-PCR, Expressing, Control

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: The effect of therapy on TRM in psoriatic lesions

doi: 10.5114/ada.2021.113125

Figure Lengend Snippet: The immunoreactive area (%) of CD8 (x ± SEM) (A), CD4 (x ± SEM) (B), CD103 (x ± SEM) (C), CD49 (x ± SEM) (D), CD69 (x ± SEM) (E), CXCR6 (x ± SEM) (F), IL-17A (x ± SEM) (G) and IL-22 (x ± SEM) (H) in the lesional skin of patients ( n = 5, dermis and epidermis) before (week 0) and during treatment (week 4 and week 12) with anti-interleukin-17 (anti-IL-17) in comparison with healthy controls ( n = 10, dermis and epidermis). Bars with different letters are significantly different ( p < 0.05)

Article Snippet: Subsequently, they were incubated at 4°C overnight with mouse anti-CD8 and CD4 or rabbit anti-CD103, CD69, CD49, CXCR6, IL-17 and IL-22 polyclonal antibodies (1 : 50; Biorbyt, UK).

Techniques: Comparison

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: The effect of therapy on TRM in psoriatic lesions

doi: 10.5114/ada.2021.113125

Figure Lengend Snippet: The immunoreactive area (%) of CD8 ( x ± SEM) (A), CD4 ( x ± SEM) (B), CD103 ( x ± SEM) (C), CD49 ( x ± SEM) (D), CD69 ( x ± SEM) (E), CXCR6 ( x ± SEM) (F), IL-17A ( x ± SEM) (G) and IL-22 ( x ± SEM) (H) in the lesional skin of patients ( n = 4), dermis and epidermis) before (week 0) and during treatment (week 4 and week 12) with anti-tumour necrosis factor α (TNF-α) in comparison with healthy controls ( n = 10, dermis and epidermis). Bars with different letters are significantly different ( p < 0.05)

Article Snippet: Subsequently, they were incubated at 4°C overnight with mouse anti-CD8 and CD4 or rabbit anti-CD103, CD69, CD49, CXCR6, IL-17 and IL-22 polyclonal antibodies (1 : 50; Biorbyt, UK).

Techniques: Comparison

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: The effect of therapy on TRM in psoriatic lesions

doi: 10.5114/ada.2021.113125

Figure Lengend Snippet: The immunoreactive area (%) of CD8 ( x ± SEM) (A), CD4 ( x ± SEM) (B), CD103 ( x ± SEM) (C), CD49 ( x ± SEM) (D), CD69 ( x ± SEM) (E), CXCR6 ( x ± SEM) (F), IL-17A ( x ± SEM) (G) and IL-22 ( x ± SEM) (H) in the lesional skin of patients ( n = 4, dermis and epidermis) before (week 0) and during treatment (week 4 and week 12) with methotrexate (MTX) in comparison with healthy controls ( n = 10, dermis and epidermis). Bars with different letters are significantly different ( p < 0.05)

Article Snippet: Subsequently, they were incubated at 4°C overnight with mouse anti-CD8 and CD4 or rabbit anti-CD103, CD69, CD49, CXCR6, IL-17 and IL-22 polyclonal antibodies (1 : 50; Biorbyt, UK).

Techniques: Comparison

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: The effect of therapy on TRM in psoriatic lesions

doi: 10.5114/ada.2021.113125

Figure Lengend Snippet: The skin location of CD8, CD4, CD103, CD49, CD69, CXCR6, IL-17A and IL-22 proteins (the representative sections) in the lesional skin (dermis and epidermis) for all the therapies. The proteins are marked in green (fluorescein). Magnification: 500×

Article Snippet: Subsequently, they were incubated at 4°C overnight with mouse anti-CD8 and CD4 or rabbit anti-CD103, CD69, CD49, CXCR6, IL-17 and IL-22 polyclonal antibodies (1 : 50; Biorbyt, UK).

Techniques:

Journal: Advances in Dermatology and Allergology/Postȩpy Dermatologii i Alergologii

Article Title: The effect of therapy on TRM in psoriatic lesions

doi: 10.5114/ada.2021.113125

Figure Lengend Snippet: Changes in the immunoreactive area (%) of CD8, CD4, CD103, CD49, CD69, CXCR6, IL-17A and IL-22 proteins in patients during therapy with: anti-interleukin-17 (anti-IL-17), anti-tumour necrosis factor α (anti-TNF-α), methotrexate (MTX) (week 0, 4 and 12). Bars with an asterisk show statistically significant differences (* p < 0.05)

Article Snippet: Subsequently, they were incubated at 4°C overnight with mouse anti-CD8 and CD4 or rabbit anti-CD103, CD69, CD49, CXCR6, IL-17 and IL-22 polyclonal antibodies (1 : 50; Biorbyt, UK).

Techniques:

Journal: Cell reports

Article Title: Arthritis flares mediated by tissue-resident memory T cells in the joint

doi: 10.1016/j.celrep.2021.109902

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206, Sigma-Aldrich Cat# 437R, RRID:AB_2884943), rabbit polyclonal anti-CD69 (Sigma-Aldrich Cat# HPA050525, RRID:AB_2681157), anti-CD4 (Clone EPR6855, Abcam Cat# ab133616, RRID:AB_2750883), anti-CD8 (Clone C8/144B, BioLegend Cat# 372902, RRID:AB_2650657), anti-CD45RO (Clone UCHL1, BioLegend Cat# 304202, RRID:AB_314418).

Techniques: Expressing, Recombinant, Methylation, Plasmid Preparation, Cell Isolation, Multiplex Assay, Microarray, Software

Journal: Cell reports

Article Title: Arthritis flares mediated by tissue-resident memory T cells in the joint

doi: 10.1016/j.celrep.2021.109902

Figure Lengend Snippet: (A) Representative immunofluorescence image of human RA synovium co-stained for CD3, CD8, CD45RO, CD69, and CD103 proteins and DAPI nuclear stain. Scale bar, 50 μm. Red circle indicates cells positive for all five biomarkers. Insert magnifies a circled cell; scale bar, 5 μm.

Article Snippet: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206, Sigma-Aldrich Cat# 437R, RRID:AB_2884943), rabbit polyclonal anti-CD69 (Sigma-Aldrich Cat# HPA050525, RRID:AB_2681157), anti-CD4 (Clone EPR6855, Abcam Cat# ab133616, RRID:AB_2750883), anti-CD8 (Clone C8/144B, BioLegend Cat# 372902, RRID:AB_2650657), anti-CD45RO (Clone UCHL1, BioLegend Cat# 304202, RRID:AB_314418).

Techniques: Immunofluorescence, Staining

Journal: Cell reports

Article Title: Arthritis flares mediated by tissue-resident memory T cells in the joint

doi: 10.1016/j.celrep.2021.109902

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206, Sigma-Aldrich Cat# 437R, RRID:AB_2884943), rabbit polyclonal anti-CD69 (Sigma-Aldrich Cat# HPA050525, RRID:AB_2681157), anti-CD4 (Clone EPR6855, Abcam Cat# ab133616, RRID:AB_2750883), anti-CD8 (Clone C8/144B, BioLegend Cat# 372902, RRID:AB_2650657), anti-CD45RO (Clone UCHL1, BioLegend Cat# 304202, RRID:AB_314418).

Techniques: Expressing, Recombinant, Methylation, Plasmid Preparation, Cell Isolation, Multiplex Assay, Microarray, Software